Evaluation of patulin contamination of apple juice, using high performance liquid chromatography [articol]

Abstract

Patulin is a mycotoxin produced by molds Penicillium patulum, Penicillium expansum, Penicillium urticae, Penicillium claviforme, Aspergillus clavatus and Byssochlamys spp., with toxically effects on human and animal body. Patulin is stable, especially in apples processed products: apples juice, apples concentrate juice, apples nectar, fruits nectar (which also has as ingredient the apples juice), apples piuree, apples in syrup, etc. In the paper are presented results of performed researches for patulin determination from apple juice, through high performance liquid chromatography. It was achieved an inhouse study for validation of method for patulin determination from apples juice, by high performance liquid chromatography. In concentration range 6.25 – 400 μg/L, the average recovery is 90.50%. Limit of detection (LOD) is 2.91 μ g/L, and limit of quantification (LOQ) is 9.71 μ g/L. The contamination degree of apple juice from commerce was evaluated using high performance liquid chromatography. Patulin concentrations of apple juice samples analyzed were under 50 μg/L, the maximum allowed limit by the legislation in force.